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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Gastroenterology. 2014 Jul 16;147(4):870–881.e8. doi: 10.1053/j.gastro.2014.07.005

Figure 4. Higher polyfunctionality of HCV-specific CD8 T cells from SR/SR patients in comparison to SR/CI patients during reinfection.

Figure 4

PBMCs were stimulated overnight with overlapping peptide pools representing HCV (NS3 and NS5B) and HCV pools showing the highest response in the IFNγ ELISPOT. ICS was performed to measure the production of IFNγ, IL-2 and TNFα and expression of CD107a. Representative results from two SR/SR patients (A, B) and two SR/CI patients (C and D), gated on CD8+ CD3+ viable T lymphocytes. Peptide pools are indicated between brackets and represent the following regions: NS3-1 (NS3 aa: 1016–1341), NS5A-2 (NS5A; aa: 2192–2426) and NS5B-1 (NS5B; aa: 2416–2720). For polyfunctionality, orange represents triple functional cells, green represents double functional and yellow represents monofunctional cells of any combination.