Table 3.
Cytokine Levels (ng/mL, mean ± 1 SD) according to estrous stage and treatment in female BALB/c mice after CLP
| Proestrus |
Estrus |
Metestrus |
Diestrus |
|||||
| Control | Buprenorphine | Control | Buprenorphine | Control | Buprenorphine | Control | Buprenorphine | |
| Plasma, 12 h | ||||||||
| IL6 | 12.32 ± 17.57 | 14.62 ± 21.90 | 7.62 ± 9.94 | 2.96 ± 5.80 | 17.1 ± 20.09 | 10.84 ± 16.48 | 5.98 ± 6.25 | 12.88 ± 23.64 |
| IL10 | 6.37 ± 10.60 | 6.14 ± 10.33 | 4.56 ± 5.67 | 1.34 ± 1.40 | 7.19 ± 8.28 | 4.9 ± 7.71 | 2.51 ± 2.03 | 5.79 ± 8.34 |
| CXCL1/KC | 0.23 ± 0.26 | 0.22 ± 0.25 | 0.28 ± 0.37 | 0.05 ± 0.05 | 0.29 ± 0.46 | 0.32 ± 0.45 | 0.19 ± 0.21 | 0.21 ± 0.39 |
| CXCL2/MIP2α | 0.30 ± 0.22 | 0.36 ± 0.25 | 0.40 ± 0.49 | 0.18 ± 0.14 | 0.36 ± 0.47 | 0.39 ± 0.40 | 0.27 ± 0.16 | 0.23 ± 0.26 |
| Plasma, 24 h | ||||||||
| IL6 | 1.87 ± 1.54 | 2.01 ± 3.30 | 1.01 ± 1.07 | 0.88 ± 0.70 | 2.73 ± 4.16 | 1.28 ± 2.03 | 5.96 ± 10.17 | 2.55 ± 3.00 |
| IL10 | 1.32 ± 0.83 | 0.52 ± 1.17a | 0.75 ± 0.76 | 0.72 ± 1.27 | 1.99 ± 2.46 | 0.95 ± 0.88 | 3.29 ± 4.6 | 1.42 ± 1.60a |
| CXCL1/KC | 56.38 ± 70.53 | 7.52 ± 5.63 | 9.29 ± 9.76 | 26.18 ± 35.89 | 54.07 ± 89.80 | 12.99 ± 13.68 | 37.67 ± 59.94 | 46.19 ± 66.68 |
| CXCL2/MIP2α | 1.93 ± 1.90 | 1.64 ± 1.95 | 1.31 ± 0.85 | 1.32 ± 1.26 | 3.90 ± 5.16 | 1.84 ± 1.94 | 4.37 ± 4.78 | 3.19 ± 5.36 |
| Peritoneum, 12 h | ||||||||
| IL6 | 6.94 ± 5.13 | 11.54 ± 6.20 | 8.84 ± 7.89 | 5.78 ± 5.83 | 8.98 ± 11.58 | 8.86 ± 11.63 | 8.58 ± 5.83 | 8.76 ± 10.41 |
| IL10 | 1.08 ± 1.25 | 0.92 ± 0.86 | 1.29 ± 1.80 | 0.63 ± 0.67 | 0.89 ± 0.82 | 0.76 ± 1.21 | 0.75 ± 0.71 | 0.95 ± 1.01 |
| CXCL1/KC | 0.01 ± 0.01 | 0.03 ± 0.03 | 0.04 ± 0.05 | 0.01 ± 0.01 | 0.03 ± 0.03 | 0.03 ± 0.04 | 0.02 ± 0.02 | 0.03 ± 0.05 |
| CXCL2/MIP2α | 0.07 ± 0.05 | 0.08 ± 0.06 | 0.06 ± 0.05 | 0.07 ± 0.03 | 0.11 ± 0.03b | 0.04 ± 0.04b | 0.08 ± 0.04 | 0.08 ± 0.07 |
| Peritoneum, 24 h | ||||||||
| IL6 | 0.82 ± 0.82 | 2.11 ± 3.69 | 0.26 ± 0.22c | 0.53 ± 0.81 | 1.72 ± 4.20 | 0.57 ± 1.25 | 1.45 ± 2.04c | 1.25 ± 1.80 |
| IL10 | 0.45 ± 0.34 | 0.33 ± 0.40 | 0.29 ± 0.36d | 0.58 ± 0.71 | 0.71 ± 0.85 | 0.28 ± 0.33 | 0.98 ± 0.92d | 0.61 ± 0.58 |
| CXCL1/KC | 2.91 ± 3.50 | 11.21 ± 17.25 | 1.04 ± 1.77 | 2.75 ± 7.52 | 5.88 ± 9.66 | 1.45 ± 2.92 | 4.90 ± 6.04 | 7.84 ± 15.35 |
| CXCL2/MIP2α | 0.74 ± 0.68 | 0.71 ± 1.28 | 0.33 ± 0.20 | 0.34 ± 0.26 | 0.58 ± 0.64 | 0.80 ± 1.44 | 0.67 ± 0.75 | 1.12 ± 2.24 |
a,b,c,d
P < 0.05 for each pairwise comparison