Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 22;5:499. doi: 10.3389/fmicb.2014.00499

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Thoma, Sepulveda, Latus and Muth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Electrophoretic mobility shift assays demonstrating binding of SpdA2-His to the palindromic sequence motif sps. Different PCR fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR_pSVH1) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A). Whereas SpdA2-His did not interact with the FA2-sps fragment (B), SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C). SpdA2-His also bound to the PCR fragment FSpdA_up, containing a T insertion within the palindromic sequence sps (D), but did not recognize FSpdA, lacking sps (E).