Table 1.
Strains and plasmids used in this work.
| Plamid | Characteristics | References |
|---|---|---|
| S. lividans TK54 | his-2, leu-2, spc-1 | Hopwood et al., 1983 |
| S. lividans TK64 | pro-2, str-6, | Hopwood et al., 1983 |
| S. lividans TK64::pSET152 | pro-2, str-6, aac(IV)3 | This work |
| E. coli XL1blue | endA1, gyrA96(nalR), thi-1, recA1, relA1, lac, glnV44, F′[::Tn10 proAB+, lacIq, Δ (lacZ)M15], hsdR17(r−K m+K) | Bullock et al., 1987 |
| BL21(DE3) pLysS | F−, ompT, gal, dcm, lon, hsdSB(r−B m−B) λ (DE3), pLysS(cmR) | Merck, USA |
| pEB211 | Fusion of pSVH1 with pK18 via NheI sites | Reuther et al., 2006 |
| pIJ101 | Conjugative Streptomyces plasmid | Kieser et al., 1982 |
| pIJ303 | pIJ101 derivative with a thiostrepton resistance gene | Kieser et al., 1982 |
| pIJ303ΔspdA2 | pIJ303 derivative with a spdA2 deletion | This work |
| pIJ303spdA2c | pIJ303Δ spdA2 complemented with spdA2 in NdeI/HindIII sites | This work |
| pIJ303-sps | pIJ303Δ spdA2 complemented with spdA2-sps in NdeI/HindIII sites | This work |
| pJOE2775 | E. coli expression vector, bla, Prham promoter, C-terminal His-tag | Altenbuchner, personal communication |
| pSET152 | Integrative pMB1derivative, PhiC31 int, attP, aac(IV)3, | Bierman et al., 1992 |
| pSSH01 | pJOE2775 with spdA2 cloned in NdeI/BamHI | This work |
| pSSH02 | pMK-RQ with spdA2-sps | This work |