Table 2.

Oligonucleotid primer used in this work.

Name	Sequence*
SpdA2-101Nde	AACATATGAGCACCACCACC
SpdA2-101Bam	AAGGATCCAGGGGTTTGCGGGTC
UpspdA101	CACTTCGCACTAACTC
Sps1-101rev	GAAGGCTGCTGCATTT
SpdA+1	GGCATATGACGGCTAGGGTCG
SpdAloB	AAAGATCTCGCGTCCGGCTGCCCCTG
SpdA-400	AAGAATTCAATATTGAAGACAGGAGAGGA
TraRNde	AACATATGACTTCGACACAGAGC
TraRhis	AAAAGCTTTCAGTGGTGGTGGTGGTGGTGGACCTCC
	AGCTCGTA
303DelAu	AGGATCTAGAGGCGCCCGCCCTCGAA
303DelAl	TGCTCATATGAATTCACCGTACGCGGCACG
303DelBu	GCCCGGTACCAAGCTTACCCGCAAACCCCTT
303DelBl	TGGCCTCGAGAGGGACGCGGGCGA
tsr-146fw	AAGATCGTCGGGAACATCGG
tsr-146rev	ACGGGAAGGGAGAAGACGTA
Slxre-146up	TCCAGGCGAGTCTTCCAGTA
Slxre-146lo	ACACGGTGATGTACGAGCAT

^*Bold letters indicate restriction sites.