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Salivary duct ligation induced an inflammatory response and necrosis. HE staining of a non-ligated submandibular gland (a) with the typical physiological appearance of a normal submandibular gland and (b) a ligated salivary gland showing histological changes following surgical duct ligation for 7 days. Cell nuclei are decentralized, inflammatory cell infiltrate is visible and thickening of the basal lamina can be observed. Magnification ×40. HE, haematoxylin–eosin.
