Diminution in mitochondrial content during hepatic remodeling. Hepatocytes were cultured in complete growth medium for 1, 2, 3 and 5 days, labeled with TMRM and imaged, as described in Materials and Methods. Single confocal images are representative of 10 or more experiments. For each culture day, total mitochondrial number per cultured hepatocyte was quantified from stacks of images through the entire thickness of cells. Values are means ± S.E.M (n = 10). *p < 0.001 compared to Day 1.