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Cytochrome c oxidase activity and mtDNA content of cultured hepatocytes. Cytochrome c oxidase activity (A) and mtDNA content normalized to nDNA (B) were assayed, as described in Material and Methods. In (B), cultured hepatocytes were treated with 0.5 µg/ ml ethidium bromide (EtBr) on Day 1 or Day 2, as indicated. *p < 0.001 compared to Day 1 in (A) and p < 0.01 compared to no treatment (None) in (B).
