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. 2014 Sep 22;4:6432. doi: 10.1038/srep06432

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(A) Schematic of in vitro differentiation of VASA:GFP transfected pluripotent stem cells across genotypes for seven days with BMP 4/8 and RA. GFP+ cells were sorted and then purity sorted each day for single cell qRT-PCR analysis. (B) Heatmap of single GFP+ cells from h9 hESC, Control iPSCs, TSC1 iPSCs and Triple X iPSCs throughout the seven day directed differentiation. Global Log₂Ex values (fold change above background) of germ cell genes is shown with grey indicating no detectable expression.