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Sequences of PCR primers used to evaluate relative expression of AgRP and MC4R genes in rat
| Amplicon length (bp) | Primer sequence | Primer |
|---|---|---|
| 181 | 5` TGGGTGTCATAAGCCTGTTGG 3` 5` GCGTCCGTGTCCGTACTG 3` |
MC4R-F MC4R-R |
| 189 | 5` TGAAGAAGACAGCAGCAGACC 3` 5` TGAAGAAGCGGCAGTAGCAC 3` |
AgRP-F AgRP-R |
| 112 | 5` AAGAAGGTGGTGAAGCAGGCATC 3` 5` CGAAGGTGGAAGAGTGGGAGTTG 3` |
GAPDH-F GAPDH-R |
