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. Author manuscript; available in PMC: 2015 Sep 18.
Published in final edited form as: Mol Cell. 2014 Sep 11;55(6):904–915. doi: 10.1016/j.molcel.2014.08.010

Figure 6. MAFG and its Corepressors Mediate CIMP in Other CRC Cell Lines and BRAF-Positive Human Tumor Samples.

Figure 6

(A) ChIP analysis monitoring binding of MAFG, BACH1, CHD8 and DNMT3B to representative CIMP gene promoters in VACO432 (left) and SW48 (right) cells.

(B) qRT-PCR analysis monitoring expression of representative CIMP genes in VACO432 (left) or SW48 (right) cells expressing a NS, MAFG, BACH1, CHD8 or DNMT3B shRNA.

(C) PAT-ChIP analysis monitoring binding of MAFG to representative CIMP gene promoters in matched adjacent normal (N) and BRAF-positive CRC human tumor (T) samples.

(D) Soft agar assay measuring colony formation of RKO cells expressing a NS, MAFG or CHD8 shRNA.

(E) Tumor formation assay. RKO cells expressing a NS, MAFG or CHD8 shRNA were subcutaneously injected into the flanks of nude mice (n=3), and tumor formation was measured. Data are represented as mean ± SD. *P≤0.05, **P≤0.01. See also Figure S6.