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. Author manuscript; available in PMC: 2015 Sep 18.
Published in final edited form as: Mol Cell. 2014 Sep 11;55(6):904–915. doi: 10.1016/j.molcel.2014.08.010

Figure 7. Oncogenic BRAF and KRAS Direct the Assembly of Distinct Repressor Complexes on Common CIMP Gene Promoters.

Figure 7

(A and B) qRT-PCR analysis monitoring CIMP gene expression in RKO (A) and DLD-1 (B) cells expressing a NS, MAFG, CHD8, ZNF304 or KAP1 shRNA. Comparable analysis of BACH1, DNMT3B, SETDB1 and DNMT1 shRNAs is shown in Figures S7B and S7D.

(C and D) ChIP analysis monitoring binding of MAFG, CHD8, ZNF304 and KAP1 on CIMP gene promoters in RKO (C) and DLD-1 (D) cells. Comparable analysis of BACH1, DNMT3B, SETDB1 and DNMT1 is shown in Figures S7E and S7F.

(E and F) ChIP analysis monitoring binding of DNMT3B to CIMP gene promoters in RKO cells (E) and binding of DNMT1 to CIMP gene promoters in DLD-1 cells (F) expressing a NS, MAFG, CHD8, ZNF304 or KAP1 shRNA. Comparable analysis of BACH1, DNMT3B, SETDB1 and DNMT1 is shown in Figures S7G and S7H. Data are represented as mean ± SD. *P≤0.05, **P≤0.01.

(G) Model for BRAF(V600E)-directed recruitment of MAFG and its corepressors to MLH1 and CIMP gene promoters. See also Figure S7.