. Author manuscript; available in PMC: 2015 Mar 18.

Published in final edited form as: Nat Commun. 2014 Sep 18;5:4896. doi: 10.1038/ncomms5896

Table 3. Thermodynamic parameters for CaM interaction with the Na_V1.2 CTD.

Titrant	N	Cell	K_d (nM)	ΔH (kcal/mol)	ΔS (cal·mol^-1·deg^-1)	N value
apoCaM	3	WT	36 ± 6	-4.54 ± 0.1	18.6 ± 0.2	1.02 ± 0.02
Ca²⁺/CaM	3	WT	1713 ± 333	6.01 ± 0.5	47.0 ± 1.3	0.76 ± 0.02
Ca²⁺/CaM₃₄	3	WT	388 ± 22	-13.85 ± 0.4	-17.9 ± 1.4	0.86 ± 0.05
apoCaM	3	R1902C	958 ± 80 ^*	-3.28 ± 0.3	-2.1 ± 0.2	0.58 ± 0.13
Ca²⁺/CaM	3	R1902C	1510 ± 187	6.19 ± 0.9	48.0 ± 2.8	0.74 ± 0.03
Ca²⁺/CaM₃₄	3	R1902C	2931 ± 123 ^*	-12.53 ± 0.9	17.4 ± 2.8	0.49 ± 0.02

P < 0.05 compared to WT.

Table 3. Thermodynamic parameters for CaM interaction with the NaV1.2 CTD.