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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925

Fig. 1. CRY2olig undergoes rapid clustering with light.

Fig. 1

(a) HEK293 cells expressing CRY2olig-mCh pre and post blue light (25 ms pulse, 488 nm, 5% laser power). Graph at right shows the relative fluorescence intensity under the line in light vs dark. Scale bar, 7.5 μm. (b) Percentage of cytosolic CRY2olig-mCh depleted into clusters after light (25 ms pulse, 488 nm), correlated with mCh expression level (A.U., arbitrary units). (c) Graph showing dependency of half-maximal clustering time of CRY2olig-mCh on cellular expression level. (d) Light dose dependence. HEK293 cells expressing CRY2olig-mCh were exposed to 2.5 – 40 ms pulses of blue light (5% laser power), allowed to cluster, then illuminated with a saturating light pulse to induce maximal clustering. (e) Clustering recovery quantification. HEK293 cells expressing CRY2olig-mCh were exposed to light at time t=0 (first arrow), followed by a dark incubation for indicated times in the presence of 35 μg/ml cycloheximide. A second pulse of light applied after recovery at 160 min (second arrow) induced re-clustering.

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