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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925

Fig. 2. LINC co-clustering assay to detect protein interactions.

Fig. 2

(a) Schematic describing LINC. (b) Positive control. COS-7 cells expressing homer1c-GFP co-cluster with homer1c-CRY2olig-mCh with blue light (50 ms pulse, 488 nm). Scale bar, 7.5 μm. (c) Negative control. PSD95-GFP does not co-cluster with homer1c-CRY2olig-mCh. Scale bar, 7.5 μm. (d) Assessment of dynamic interactions between CaMKII and CaM in response to Ca2+. COS-7 cells expressing CIBN-mCh-CaMKII, CRY2olig, and CaM-YFP were imaged pre and post blue light exposure (500 ms pulse, 488 nm). At resting Ca2+, only a small amount of CaMYFP colocalizes with CaMKII clusters. Addition of ionomycin elevates intracellular Ca2+, resulting in increased CaM-YFP localized to clusters. Scale bar, 5 μm.

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