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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925

Fig. 3. LINC-FRAP assay.

Fig. 3

(a) Schematic describing use of LINC-FRAP in synapses. Mobility of a CRY2olig-fused protein (blue) is restricted after light-induced clustering, which restricts the mobility of an interacting FP-tagged protein (red). (b) LINC-FRAP control experiments in primary neuronal cultures showing exchange of CRY2olig-mCh-homer1c is delayed at the indicated photobleached synapse (arrowhead) after blue light clustering. (c) LINC-FRAP quantification before and after blue light, testing homer/PSD95 and homer/homer interactions at synapses.

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