Table 2. The effect of a conditional BSP knockdown on soft tissue and osteolytic lesions as investigated in nude rats inoculated with MDA – MB – 231 cell clones B3 and B7 into the saphenous artery.
| Cell clone | Period of doxycycline intake (weeks) | Period of treatment by miBSP (weeks) | Volume [μl] of soft tissue lesion (range) | T/C* 100 (soft tissue lesion)a) | Volume [μl] of osteolytic lesion (range) | T/C* 100 (skeletal lesion)a) |
|---|---|---|---|---|---|---|
| B 3 | 4 | 0 | 153.7 b) (6.7 - 403.4) | 100 | 75.4 153.7 b) (0.2 - 206.9) | 100 |
| 2 | 2 | 62.8 (0 – 182.2) | 41 | 11.3 (0 - 34.0) | 15 | |
| 1 | 3 | 43.2 153.7 b) (0 - 121.4) | 28 | 5.1 153.7 b) (0 - 13.2) | 6.7 | |
| B 7 | 4 | 0 | 76.5 153.7 b) (12.2- 244.1) | 100 | 26.5 153.7 b) (0.3 - 101.2) | 100 |
| 2 | 2 | 70.6 (0 - 211.8) | 92 | 22.9 (0 - 68.0) | 86 | |
| 1 | 3 | 11.0 153.7 b) (0 - 30.6) | 14 | 2.4 153.7 b) (0 - 4.5) | 9 |
a)
Mean tumor or skeletal lesion volume of treated (T) over untreated control (C) rats times 100;
b)
After 3 weeks of miRNA exposure, the soft tissue and osteolytic lesions were decreased significantly in treated versus control groups (p < 0.03), according to a pooled analysis with the two-tailed Wilcoxon test.