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. 2014 Jul 15;5(14):5832–5844. doi: 10.18632/oncotarget.2200

Figure 1. HERG1 was highly expressed both in pancreatic tumor tissues and cell lines.

Figure 1

(A) Immunohistochemical staining of HERG1 protein in pancreatic cancer, magnification, ×200: (a) negative control (PBS) (b) poorly differentiated pancreatic tumor tissues with positive HERG1 immunohistochemical reaction (c) well differentiated pancreatic tumor tissues with positive HERG1 immunohistochemical reaction (d) adjacent normal tissues negative for HERG1; (B) Semiquantitative RT-PCR analysis of HERG1 mRNA expression in pancreatic cancer cell lines: (a) agarose gel representing HERG1 mRNA positive (PANC-1, CFAC-1, SW1990, BxPC-3 and SGC-7901) and negative (HPAC and GES-1) mRNA expression (b) HERG1 mRNA expression in pancreatic cancer cell lines relative to the average HERG1 mRNA expression in normal pancreatic tissue adjacent to tumors; (C) Western blot analysis of HERG1 protein expression in pancreatic cancer cell lines: PANC-1, CFAC-1, SW1990, BxPC-3 and SGC-7901 with positive and HPAC and GES-1 with negative protein expression.