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. 2014 Jul 15;5(14):5832–5844. doi: 10.18632/oncotarget.2200

Figure 3. HERG1 is a direct target of miR-96.

Figure 3

(A) Influence of miRNAs predicted to target HERG1 on luciferase reporter activity upstream of the HERG1 3'-UTR region. (B) The nucleotide sequence of miR-96 binding sites within the HERG1 3'-UTR region and a schematic diagram of the reporter constructs showing the entire HERG1 3'-UTR sequence (wild type, HERG1-WT and mutant type, HERG1-MUT); mutated nucleotides in miR-96 binding site are underlined. (C) HERG1-WT and the HERG1-MUT reporter luciferase activity in HEK-293 cells treated with pre-miR-96 or miR-96 inhibitor (50 nM), or without treatement. (D) HERG1-WT luciferase reporter activity in PANC-1 and CFPAC-1 after treatment with 50 nM of pre-miR-96, or without treatement. (E) HERG1 mRNA and protein expression in PANC-1 and CFPAC-1 cells treated with pre-miR-96 or miR-96 inhibitor, or without treatement. (a) Western blot (b) RT-PCR. All data are shown as mean ± SD.