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. 2014 Jul 15;5(14):5832–5844. doi: 10.18632/oncotarget.2200

Figure 5. Overexpression of miR-96 inhibits pancreatic cancer cell growth, migration, invasion in vitro, suppresses tumorigenicity, metastasis and contrasts with HERG1 expression in vivo.

Figure 5

(A) plasmid induced overexpression of miR-96 in PANC-1 and CFPAC-1 cells; (B) PANC-1 and CFPAC-1 growth curve after transfection with pre-miR-96 plasmid or empty vector or without treatment. The growth index was assessed at 1, 2, 3, 4, and 5 d; (C) migration and (D) invasion assay analysis of PANC-1 and CFPAC-1 cells after transfection with pre-miR-96 plasmid or empty vector or without treatement. (E) The growth curve (a) and weight of tumors (b) derived from untreated, empty vector and miR-96 plasmid-transfected CFPAC-1 cells were injected into the left flank of 4-week-old female BALB/c nu/nu mice. (F) Immunohistochemical analysis of HERG1, in tumors derived from miR-96 overexpression, untreated and empty vector nude mice groups. (G) Metastatic nodules (a) and H&E staining (b) derived from untreated, empty vector and miR-96 plasmid-transfected CFPAC-1 cells injected into the pancreatic capsule of 4-week-old female BALB/c nu/nu mice. Magnification, ×40. All data are shown as mean ± SD. * P < 0.05; ** P < 0.01.