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. 2014 Jun 20;5(14):5472–5482. doi: 10.18632/oncotarget.2121

Figure 6. Glo-1 expression and activity in human breast cancer cells upon MG stimulation.

Figure 6

Breast cancer cells were treated with MG at 300, 500 or 1000 μM for 6 hours. Only triple negative cancer cells are capable to respond to MG treatment by up-regulating both Glo-1 expression (A) and activity (B). No modulation of Glo-1 was observed in the other cell subtypes analyzed. Beta-actin expression was used to normalize the amount of loaded proteins. Semi-quantitation analysis was done using beta-actin as a loading control. Results are the average of independent experiments (n= 3). Data were statistically analyzed with two-way ANOVA followed by Bonferroni multiple comparisons (* p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 vs untreated cells).