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. 2014 Jun 26;5(14):5602–5614. doi: 10.18632/oncotarget.2136

Fig.4. Overexpression of NUMB-1 suppresses KYSE150 cell growth by arresting cells in M phase.

Fig.4

(A) Cell cycle analysis by flow cytometry was performed to determine the change in cell cycle distribution of KYSE150 NUMB-1 overexpressors compared to KYSE150 control cells. The percentage of KYSE150 cells overexpressing NUMB-1 found in the G2/M phase (40.04±1.07%) was significantly higher than the percentage of control KYSE150 cells (24.34±0.57%). (B) A similar result was observed in KYSE30 cells with the G2/M ratio increasing from 22±3.6% in control cells to 38±7.81% in NUMB-1 overexpressors. (C) Representative images of H&E staining show an increase in polyploidy (indicated by the arrows) induced by overexpressing NUMB-1 in KYSE150 cells. (D) Western blot for caspase-3 and PARP expression demonstrate that Numb-1 could not induce caspase-3 and PARP cleavage in KYSE150 cells. The positive control was HL60 cells treated with 10 μM DDP. (E) Overexpression of NUMB-1 significantly suppressed foci formation in both KYSE150 (164±18.1 vs 80.3±12.8) and KYSE180 cells (465±42 vs 223±26) compared to control-infected cells.