Figure 4. Decreased expression of KISS1R, KSR1, CAMK1, and SSPN leads to tumor cell death and/or senescence.

(A) Protein expression analysis of KISS1R, KSR1, CAMK1, and SSPN in NRE and ccRCC cell lines via western blot. VHL mutation status is provided. (B) QPCR for mRNA transcript expression in NT and hit knockdown cells are presented as fold change relative to NT control, where NT values are set at 1. (C) Proliferation of NT vs. hit targeted shRNA cell lines. (D) Western blot for protein expression in NT control and shRNA targeted cells for PARP, and P21. β-actin was used as a loading control. (E) Flow cytometry analysis of propidium iodide stained NT and target knockdown cells evaluating cell death. Increases of 5% or greater versus NT control cells were considered to be significant.