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. 2014 Jul 5;5(14):5725–5735. doi: 10.18632/oncotarget.2167

Figure 5. miR-142-3p regulates cancer stem cell-like properties in HCC via the direct targeting of CD133.

Figure 5

(A-B) Stable overexpression of CD133 in Huh7 cells with miR-142-3p stably overexpressed was confirmed at both genomic and proteomic levels by qPCR and Western blot, respectively. M68 stands for pReceiver-M68 negative control vector in which CD133 was cloned into. (C) Growth curve of miR-142-3p overexpressing Huh7 cells stably transfected with M68 control vector and CD133, as determined by XTT cell proliferation assay. (D) Representative images of spheroid formation, migration, invasion and capillary tube formation assays demonstrating the ability of CD133 overexpression in miR-142-3p expressing Huh7 to rescue cancer and stem cell-like properties in HCC. Magnification at 400x.