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Automated, quantitative analysis (AQUA) of histospots from high NY-ESO-1 expressing (AQUA score: 46.5) (upper-right) and low NY-ESO-1 expressing (AQUA score: 19.9) (lower-right) RCCs. Anti-cytokeratin conjugated to Cy2 was used to identify the cytoplasm and to define the tumor mask. DAPI was used to define the nuclear compartment and Cy5 was used to visualize the target (NY-ESO-1).
