Fig. 4.

Desphosphorylation of STAT3, STAT5, AKT in human SET2 and CMK cells. Human SET-2 and CMK cell lines carrying JAK2^V617F or JAK2^wt respectively, were treated with equipotent doses (IC₂₀, IC₅₀, and IC₈₀) of atiprimod for 20 h. Whole cell lysates were separated on a 4–12% SDS-PAGE gel, and western blotting was performed using mouse monoclonal anti-p-STAT3, anti-p-STAT5, and anti-p-AKT. After stripping, the membrane was reprobed with rabbit anti-total STAT3, anti–total STAT5, and anti-total AKT. The quantity of protein was confirmed by probing the membranes with β-actin