Skip to main content
. 2014 Sep 3;193(7):3600–3612. doi: 10.4049/jimmunol.1401088

FIGURE 2.

FIGURE 2.

Opposing effects of exogenous IFN-β treatment and autocrine type I IFN signaling on IL-12 and TNF-α production in M. tuberculosis–infected macrophages. (A) WT macrophages were infected with M. tuberculosis in the presence of increasing concentrations of IFN-β (for protein) or 2 ng/ml IFN-β (for mRNA), added at the time of infection, and levels of IL-12p40, IL-12p70, and TNF-α protein in supernatant and Il12b, Il12a, and Tnfa mRNA from cells were determined by ELISA at 24 h postinfection and qRT-PCR at indicated times after infection. (B) IL-1β protein and Il1b mRNA from macrophages in (A) were measured by ELISA at 24 h postinfection and qRT-PCR at indicated times after infection. (C) WT and Ifnar1−/− macrophages were infected with M. tuberculosis and IL-12p40, IL-12p70, and TNF-α protein in supernatant and Il12b, Il12a, and Tnfa mRNA from cells were determined by ELISA at 24 h postinfection and qRT-PCR at indicated times after infection. (D) IL-1β protein and Il1b mRNA from macrophages in (C) were measured by ELISA at 24 h postinfection and qRT-PCR at indicated times after infection. Graphs show means ± SEM of triplicate samples. For ELISA results, uninfected control samples were below the detection limit (20 pg/ml) for the cytokines measured (data not shown). Significance was determined using an unpaired t test. Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.