FIGURE 6.

Type I IFN regulation of IL-1β production is dependent on iNOS and IL-10. WT and Nos2^−/− macrophages were infected with M. tuberculosis, treated with anti–IL-10R or isotype control Abs and with either IFN-γ (5 ng/ml) or IFN-β (2 ng/ml) or media, added at the time of infection. Levels of the indicated cytokines in culture supernatant were determined at 24 h postinfection by ELISA. Uninfected control samples were below the detection limit (20 pg/ml) for the cytokines measured (data not shown). Graphs show means ± SEM of triplicate samples. Data are representative of at least three independent experiments.