Skip to main content
. Author manuscript; available in PMC: 2014 Sep 22.
Published in final edited form as: Nat Protoc. 2013 Aug 15;8(9):1730–1742. doi: 10.1038/nprot.2013.104

Figure 6.

Figure 6

Bioactive peptide degradation in mouse spinal cord and plasma. A. LC-MS profile of multiple-charged endogenous CGRP1–37 and heavy-labeled CGRP1–37 in the RAPID method64 B. IDE regulates CGRP levels in mice as demonstrated by elevated levels of CGRP in animals lacking IDE. Capsaicin causes CGRP secretion leading to higher absolute levels of CGRP but the difference in CGRP levels between genotypes is consistent indicating that IDE can regulate CGRP under different conditions. C. CGRP proteolysis pathway revealed through the application of this protocol.