Figure 9. Driselase digestion products of pAMAC-labelled •OH-attacked pectin: electrophoretic analysis.
(a) De-esterified pre-reduced citrus pectin was successively treated with •OH, AMAC, acetone and finally Driselase for 7 and 14 days; the products were then fractionated on a mini C18 column eluted with 0–40% methanol. The image shows electrophoretograms at pH 6.5 of the fluorescent fractions. (b) As in (a) but each fraction was de-lactonized before electrophoresis. Markers a and b are identical mixtures before and after de-lactonization.