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Western blot analysis of S2VP10, S2CP9, Panc-1, and Miapaca-2. Metastatic pancreatic cancer cell lines (S2VP10, S2CP9) in vitro demonstrated up-regulation of IGF1-R compared to non-metastatic pancreatic cancer cell lines (Panc-1, Miapaca-2). The downstream signals of IGF1-R, including GRB2 and PI3K, showed increased protein expression in metastatic pancreatic cancer cell lines.
