Figure 4.

Effect of cytotoxicity of SC-CO₂ extracts of O. stamineus. Photomicrographic images of PC3 and MDA-MB-231 cells taken under an EVOS f1 digital microscope at 20x magnification after 48 hours of treatment with the B2. The vehicle (0.1% DMSO) treated cells showed fully confluent growth with compact layer of highly proliferating HCT 116 cells. The cells displayed well established pseudopodial cellular projections with prominent nuclei, whereas the extracts (B2, C1, C2, and C3) treated cells demonstrated remarkable inhibitory effect on proliferation of both cell types. The pictures revealed that the population of cells reduced drastically within the 48 hours of treatment. In addition, the extracts induced noticeably morphological changes in the cells.