Fig. 2.

Rates of DNA cleavage with various concentrations of three different substrates. The ΔQNK-FN concentration in the digests was fixed at 16 nM. The substrate concentrations were varied depending on the substrate: pKT8 (2, 4, 8, 12, and 24 nM); pKT28 (2, 4, 8, 12, and 16 nM); and pKS (2, 4, and 8 nM). The reaction was performed in buffer containing 50 mM NaCl. The rates are plotted versus the substrate concentrations for each substrate: pKT8 (circles), pKT28 (squares), and pKS (triangles).