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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Bioorg Med Chem. 2013 Dec 24;22(18):5097–5109. doi: 10.1016/j.bmc.2013.12.030

Table 3.

Inhibition of tubulin polymerization and colchicine binding by compounds 1, 2a–c, 2f–h and 2j

Compound Tubulin assemblya,c IC50 ± SD
(µM)		 Colchicine bindingb,c
% ± SD
2a 1.9 ± 0.02 52 ± 0.9
2b 3.1 ± 0.2 46 ± 0.9
2c 0.74 ± 0.06 84 ± 5
2f >20 n.d.
2g 10 ± 0.7 n.d.
2h >20 n.d.
2j 16 ± 1 n.d.
1 2.0 ± 0.06 52 ± 4

n.d. = not determined.

a

Inhibition of tubulin polymerization. Tubulin was at 10 µM.

b

Inhibition of [3H]colchicine binding. Tubulin, colchicine and tested compound were at 1, 5 and 5 µM, respectively.

c

Values are mean ± SD of four separate experiments.