Table 1.
Oligonucleotide Primer Pairs Used in Polymerase Chain Reaction Amplifications for the Detection of Bartonella Species in Bats and Bat Flies
| Primer pairs | 5′-primer sequence-3′ | Target locus | Amplicon (bp) | Reference |
|---|---|---|---|---|
| BhCS781.p BhCS1137.n CSH1fa BhCS1137.na |
GGGGACCAGCTCATGGTGG AATGCAAAAAGAACAGTAAACA GCGAATGAAGCGTGCCTAAA AATGCAAAAAGAACAGTAAACA |
Citrate synthase gene (gltA) |
379 350 |
Norman et al. 1995 Birtles and Raoult 1996 |
| 600f 800r |
CGTGCAACAGAAGATTTAGATC CGATTCGCATCATCATTTTC |
RNA polymerase beta subunit gene (rpoB) | 191 | Morick et al. 2009 |
| 1400f 2300r |
CGCATTGGCTTACTTCGTATG GTAGACTGATTAGAACGCTG |
RNA polymerase beta subunit gene (rpoB) | 830 | Renesto et al. 2001 |
| 321s 493as |
AGATGATGATCCCAAGCCTTCTGG TGAACCTCCGACCTCACGCTTATC |
16S-23S (ITS) | 208 | Maggi et al. 2005 |
a
Primer pair used for amplification of gltA sequences from DNA extracted from bat blood and flies.