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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Obesity (Silver Spring). 2013 Sep 10;22(2):380–389. doi: 10.1002/oby.20535

Fig. 8. GHSR1a expression in C2C12 cells and mouse pituitaries.

Fig. 8

RNA was extracted from C2C12 cells treated with vehicle or increasing doses of ghrelin and real time PCR was performed using specific primers for GHSR1a. Expression was quantified relative to HPRT (A) and amplicon size was further verified on an agarose gel (B).