Table 1. List of Primers used in construction of chiA2 deleted V. cholerae strain and cloning chiA2 in pBAD-TOPO TA expression vector.

Primers	Sequence
chiA2XbaI A F	5 ′-CAGCTCTAGACTCTGATACTGACGTTCGAG-3′
chiA2 B R	5 ′-CCCATCCACTATAAACTAACACAATGGCTTGAGGTGTGAAC-3′
chiA2 C F	5 ′-TGTTAGTTTATAGTGGATGGGGGAACAGGTGGTGATGCTT-3′
chiA2 SACI D	5 ′-GAATCGAGCTCGATTGCATTCCCAATGAG-3′
chiA2 external F	5 ′-GCAGCACCCTCGGCTCC-3′
chiA2 external R	5 ′-CGCGGCACCGAGGTC-3′
chiA2 expression F	5 ′-ATGAATCGAATGACTTTGTGCG-3′
chiA2 expression R	5 ′-TTAATGAGTAGAACAACTCGC-3′