Figure 2. 91R mAb recognizes the human CCR9 N-terminal domain. (A) Diagram of human and mouse CCR9 and the chimeric CCR9 bearing the human CCR9 sequence with the N-terminal domain (Nt) replaced by the murine sequence (mNt/hCCR9); flow cytometry analyses with 91R mAb (anti-hCCR9; open histograms) and isotype-matched control mAb (filled histograms), rabbit polyclonal K629 (anti-mCCR9; open histograms) and rabbit control Ab (filled histograms). (B) Membrane-enriched lysates from pCIneo-, hCCR9-HEK and MOLT-4 cells were used for western blot with 91R; anti-CD71 Ab was used as loading control. Where indicated, cell lysates were PNGase-treated to remove N-glycosylated residues. A representative experiment is shown (n = 2).