Table 1. Binding kinetics of 3.3, 1E3 and 2B5 antibodies to PEG.
| Temperature | 4°C | 25°C | 37°C | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Antibody | kon (M−1s−1) | koff (s−1) | KD (M) | kon (M−1s−1) | koff (s−1) | KD (M) | kon (M−1s−1) | koff (s−1) | KD (M) |
| 3.3 | (0.9 ± 0.12) × 103 | (4.8 ± 0.14) × 10−4 | (5.2 ± 0.50) × 10−7 | (9.6 ± 1.67) × 103 | (1.2 ± 0.70) × 10−3 | (1.3 ± 0.17) × 10−7 | (1.5 ± 0.03) × 104 | (3.1 ± 0.22) × 10−3 | (2 ± 0.11) × 10−7 |
| 1E3 | (2.1 ± 0.13) × 103 | (7.4 ± 0.44) × 10−4 | (3.4 ± 0.04) × 10−7 | (0.9 ± 0.04) × 103 | (2.8 ± 0.22) × 10−2 | (2.9 ± 0.16) × 10−5 | NMa | NM | NM |
| 2B5 | (3.6 ± 0.02) × 103 | (1.7 ± 0.01) × 10−4 | (4.7 ± 0.06) × 10−7 | (1.6 ± 0.02) × 103 | (14.2 ± 0.64) × 10−2 | (8.7 ± 0.47) × 10−5 | NM | NM | NM |
The binding of 3.3, 1E3, and 2B5 Fab fragments to PEG2k-HEL immobilized on a CM5 chip was determined in a Biacore T-200. Results represent mean values of triplicate determinations ± SD aNM, not meaningful, no reading at all concentrations.