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Figure 1. Schematic of the free human PCSK9 assay. The ELISA method utilizes an anti-human PCSK9 monoclonal that binds to the same epitope as the therapeutic antibody and a second biotin conjugated anti-human PCSK9 antibody for detection. The capture antibody by virtue of binding to the same epitope as the therapeutic should capture only the free form of PCSK9 present in samples.
