Figure 3. Parallelism of the endogenous ligand within the method was evaluated across three individual donors that were diluted at multiple dilutions and then quantitated against the recombinant PCSK9 standard curve. For each donor the concentration measured at each dilution, divided by the mean ng/mL concentration of the dilution series was assessed to determine if the ratio fell within the range of 0.8 -1.2.22 As shown all values met the acceptance criteria, giving the assay validity as a quantitative biomarker method.