Fig. 6.

Effects of anion (A-9-C or DIDS) and cation (TEA-Cl) channel blockers on the growth rate of maize coleoptile segments incubated in the presence of IAA (10 μm). Coleoptile segments were preincubated (1 h) in control medium, the channel blockers were then added to the incubation medium, and IAA was added at 2 h. Curves represent data obtained using the second measuring system. Inset on the right shows total elongation growth, calculated as the sum of extensions measured at 3-min intervals for 10 h. Inset on the left presents medium pH changes for maize coleoptile segments incubated in the presence of IAA. All curves represent means of at least seven independent experiments. Bars indicate ± s.e. The LSD test for growth is included in Supplementary Data Fig. S2.