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. 2014 Sep 22;9(9):e107197. doi: 10.1371/journal.pone.0107197

Figure 3. Titration of fluorescence by ATP.

Figure 3

Changes in the fluorescence upon addition of ATP are expressed as percent of the fluorescence before the addition of ATP. Values are taken when the fluorescence reached a plateau. Error bars represent standard errors. The solid line represents the theoretical curve with the Hill equation (Fluorescence quenching  = ΔFL max×[ATP]n/(Kd n+[ATP]n)) with the following parameters(± standard error); Kd = 36.5±1.2 µM, ΔFL max = 71.0±0.7%, n = 1.47±0.1. The dotted line represents the theoretical curve with simple binding equation (Fluorescence quenching  = ΔFL max×[ATP]/(Kd+[ATP])) with K d = 34.4±2.9 µM, ΔFL max = 73.5±1.5%.