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Inhibition of NO release from LPS stimulated BV2 microglia by nicotine. BV2 microglia were pretreated for 1h with increasing concentrations of nicotine and subsequently challenged with 10μg/ml LPS for 24h. Culture media was collected and assayed for nitrite content using the Griess reaction. NO release is expressed as percentage of NO release from LPS alone. * p<0.001 compared to LPS alone and Nic+MEC by Tukey's multiple comparison test following a one-way ANOVA.
