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Concentration response curves for the inhibition of TNF-alpha release from LPS stimulated BV2 microglia by rhamnetin (A) and with the addition of MLA (B). BV2 microglia were pretreated with increasing concentrations of rhamnetin, in the presence or absence of 10μM MLA for 1h and subsequently challenged with 10μg/ml LPS for 24h. Culture media was collected and assayed for TNF-alpha content by ELISA. TNF-alpha release is expressed as percentage of TNF-alpha release from LPS alone.
