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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Fitoterapia. 2014 Jun 24;98:11–21. doi: 10.1016/j.fitote.2014.06.012

Figure 7.

Figure 7

Concentration response curves for the inhibition of TNF-alpha release from LPS stimulated BV2 microglia by sakuranetin (A) and with the addition of nicotine (B). BV2 microglia were pretreated with increasing concentrations of sakuranetin, in the presence or absence of 1μM nicotine for 1h and subsequently challenged with 10μg/ml LPS for 24h. Culture media was collected and assayed for TNF-alpha content by ELISA. TNF-alpha release is expressed as percentage of TNF-alpha release from LPS alone.