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. 2007 Dec 14;13(46):6219–6225. doi: 10.3748/wjg.v13.i46.6219

Figure 2.

Figure 2

Oxidant status of KKU-100 cells after exposure to a mixture of inflammatory cytokines. A: Stimulation of nitric oxide production, assayed as nitrite levels. Cultured media was collected at 24 and 48 h after exposure; B: Superoxide formation. Cell cultures were washed and incubated with PMA (0.68 μg/mL), L-NAME (100 μmol/L), or NADPH (200 μmol/L), and superoxide production was measured using the chemiluminescence method; C: Redox status was assessed as the GSH and GSSG ratio. Results are presented as mean ± SE from 3 separate experiments. aP < 0.05 between cytokine treated and the respective control groups.