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. 2007 Oct 21;13(39):5208–5216. doi: 10.3748/wjg.v13.i39.5208

Figure 2.

Figure 2

A: Triple color staining of nuclei (blue-DAPI), CgA (green-Alexa 488) and CTGF (red-Cy5) in a SI carcinoid tumor from the carcinoid TMA. Staining for both CgA and CTGF was cytoplasmic. Dual-stained (CgA + CTGF) cells are yellow. A majority of CgA cells (about 80%) were also CTGF positive (x 400); B: Triple color staining of nuclei, serotonin (green-Alexa 488) and CTGF in a carcinoid tumor from the TMA. Staining for both Serotonin and CTGF was cytoplasmic. Dual-stained (Serotonin + CTGF) cells are yellow. A majority of the serotonin cells (about 95%) were also CTGF positive. (x 600); C: Triple color staining of nuclei (blue-DAPI), cytokeratin (green - Alexa 488) and TGFβ1 (red-Cy5) in a carcinoid tumor from the TMA. Staining for TGFβ1 was cytoplasmic. A majority of the carcinoid tumor cells (cytokeratin-positive) (about 85%) were also TGFβ1 positive (x 200).