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. 2014 Sep 22;9(9):e107164. doi: 10.1371/journal.pone.0107164

Figure 4. The sperm viability, as assessed by a fluorescent staining method.

Figure 4

Rat Sperm viability assessment by SYBR-14/PI staining. (A) Control rat sperm appear green due to uptake of SYBR14 only; (B) DGT-treated rat sperm appear red due to uptake of PI when observed under a fluorescence microscope. Overlaid fluorescence images of (C) control and (D) DGT-treated human spermatozoa, dual stained with SYBR-14 and propidium iodide to distinguish green-fluorescing live from red-fluorescing dead spermatozoa.