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. 2014 Sep 22;9(9):e107164. doi: 10.1371/journal.pone.0107164

Figure 5. Irritation potential as measured by hemolytic activity and effect on Lactobacillus acidophilus in vitro.

Figure 5

(A) Dose-dependent effect of DGT in rat RBCs. Isolated RBCs were incubated with varying drug dilutions, Triton X-100 and PBS. The extent of hemolysis was determined spectrophotometrically. (B) Effect of desgalactotigonin (DGT) and nonoxynol-9 on Lactobacillus acidophilus. [Optical density as the measure of turbidity denoting growth of bacterial colonies during 36 h of culture in the absence (control) and presence of DGT. There was a gradual increase in the growth of colonies that reached a plateau after 24 h of culture. Irrespective of the dose of DGT (19×MEC), the growth of bacterial colony was comparable with that of the control. N-9, however, exerted constant inhibition of bacterial growth throughout the entire culture period.]